Sphingosine Kinase 1 Inhibitor Screening Assay Kit - 96 Well

Sphingosine kinase 1 (SPHK 1) and SPHK 2 phosphorylate D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation Cayman`s SPHK 1 Inhibitor Screening Assay Kit provides a fluorescence-based method for screening SPHK 1 inhibitors. The procedure requires only two easy steps, both performed in the same microplate. In the first step, human recombinant SPHK 1 phosphorylates D-erythro-sphingosine C-18. In the second step, ADP is converted to hydrogen peroxide (H2O2) which then reacts with ADHP in the presence of peroxidase to yield the fluorescent product resorufin.Application/Inhibitor Screening Assay, Chemical Class/Assay Kits, Research Area/CancerSynonyms :SPHK 1