P-Glycoprotein Drug Interaction Assay Kit - 1 ea

P-Glycoprotein (P-gp) is an active plasma membrane transporter involved in drug pharmacokinetics and cellular detoxification. P-gp exhibits a high drug-dependent ATP hydrolysis activity that is a reflection of its drug transport ability. The test of drug stimulation or inhibition of ATPase activity may be used to screen the potential drug interaction with P-gp. This assay provides an in vitro screening method for testing drug interaction with P-gp based on the study of modulation of basal or induced ATPase activity from enriched P-gp membrane vesicle preparations. P-gp ATPase activity is measured by a spectrophotometric method based on continuous monitoring of ADP formation in the vesicle suspension medium in a 96 well plate format. Data are analyzed by comparing the variation of the basal or stimulated activities to reference activities.Chemical Class/Assay Kits, Research Area/Drug DiscoveryKeywords:glycoproteins pharmacokinetics cellular detoxification ATPase ADPase assays kits interactions drugs transport photodetection spi-bio spibio D5001Synonyms :P-gp Drug Interaction Assay Kit