Progesterone (free in saliva) ELISA - 96 Determinations

The IBL-AMERICA Salivary Progesterone ELISA kit is based on the competition principle and the microplate separation.An unknown amount of Progesterone present in the sample and a fixed amount of Progesterone conjugated with horseradish peroxidase compete for the binding sites of a rabbit polyclonal Progesterone -antiserum coated onto the wells. After one hour incubation the microplate is washed to stop the competition reaction. After adding the substrate solution, the concentration of Progesterone is inversely proportional to the optical density measured. Application: Method:ELISA Kit Species:Human Sample Type:Saliva. SStorage:2-8°C Stability: Sensitivity:3.8 pg/mL Time:1 hour (RT) + 15 min. (RT) = 1 hour, 15 min. total