NAD/NADH Assay Kit - 100 assays

Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. BioChain's NAD+/NADH assay kit is based on an alcohol dehydrogenase cycling reaction, in which a tetrazolium dye (MTT) is reduced by NADH in the presence of phenazine methosulfate (PMS). The intensity of the reduced product color, measured at 565 nm, is proportionate to the NAD+/NADH concentration in the sample. Our assay is a convenient method to measure NAD, NADH and their ratio.